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Pharmacelsus

In vitro & in vivo Toxicology Services

The Pharmacelsus service portfolio on in vitro and in vivo toxicology service portfolio offers you a complete workflow. De-risk either your early stage drug discovery or late stage development programs with us. In vitro toxicology assays are performed in-house in a quality controlled way. In vivo toxicology studies according to national and international guidelines are available on rodent and non-rodent species in cooperation with our trusted partner. In vivo non-GLP rodent studies are performed in house.

In vitro Toxicology

Cytotoxicity

  • Test systems
    Established cell lines and primary cells, 2D– monolayer, suspension and 3D-organoid cultures
  • Analysis
    Fluorescence, luminescence, absorbance
  • Indicator
    Resazurin, XTT, MTT, ATP, LDH
  • Readout
    Viability, Cell Membrane Damage, Energy content

Mitochondrial Toxicity

  • Test systems
    HepG2, HepG2-Oxphos
  • Analysis
    Fluorescence, luminescence
  • Indicator
    Resazurin, XTT, ATP
  • Readout
    Viability
  • Format
    96-Well Plate/Plate Reader
  • Multiplexing of analytical endpoints available

Metabolite Toxicity

  • Test systems
    HepG2 w/ and w/o metabolic activation by S9-fraction
  • Analysis
    Luminescence
  • Indicator
    ATP
  • Format
    96-Well Plate/Plate Reader
  • Metabolite identification via LC-MS
  • Reactive metabolite trapping in liver microsomes

Immunotoxicity

  • Test systems
    PMBC, monocytes, blood
  • Analysis
    Luminex technology
  • Readout
    Lymphocyte subsets, cytokine levels
  • Format
    96-Well Plate/Plate Reader, Luminex
  • Can be combined with other systems e.g. viability assays
  • Recommended assays according to ICH S8

Hepatotoxicity

  • Test systems
    HepG2, primary hepatocytes
  • Analysis
    Fluorescence, luminescence, Luminex technology
  • Indicator
    Resazurin, ATP
  • Readout
    Viability, Energy content, biomarker levels
  • Format
    96-Well Plate/Plate Reader, Luminex

Allergic Response / Histamine Release

  • Test systems
    Peritoneal mastocytes
  • Analysis
    Absorption
  • Indicator
    O-phtaldialdehyde
  • Readout
    Histamine release
  • Format
    96-Well Plate/Plate Reader

In vivo Toxicology*

Species

  • Rodents
  • Non-rodent species
    (please contact us)

Routes of Administration

  • Oral
  • Intraperitoneal
  • Intravenous
  • Subcutanous
  • Dermal
  • Inhalation
  • Rectal
  • Others
    (please contact us)

Acute Toxicity

  • Systemic toxicity
  • Phototoxicity
  • Local tolerance
  • Skin sensitisation and irritation
  • Others
    (please contact us)

Subchronic & Chronic Toxicity

  • Rodents and non-rodents
  • DRF studies
  • MTD studies
  • Toxicokinetics

Reproductive Toxicity

  • Multigeneration studies
  • Fertility and reproduction
  • Embryotoxicity
  • Pre/post-natal development

Pathology

  • Histology
  • Immuno- histopathology
  • others
    (please contact us)

* in cooperation. All studies are conducted according to national and international guidelines.

Further toxicological assays are available on request.

Please contact us for your specific toxicology study!

For in vitro Toxicology

Dr. Julia Susewind
In vitro Toxicology
susewind@pharmacelsus.de

For in vivo Toxicology

Anika Christoffel
Team Manager in vivo Pharmacology & Pharmacokinetics
christoffel@pharmacelsus.de

Pharmacelsus GmbH
Science Park 2
66123 Saarbruecken / Germany

Phone: +49 (0)681 3946 7510
Email:  info@pharmacelsus.de