Pharmacelsus
In vitro & in vivo Toxicology Services
The Pharmacelsus service portfolio on in vitro and in vivo toxicology service portfolio offers you a complete workflow. De-risk either your early stage drug discovery or late stage development programs with us. In vitro toxicology assays are performed in-house in a quality controlled way. In vivo toxicology studies according to national and international guidelines are available on rodent and non-rodent species in cooperation with our trusted partner. In vivo non-GLP rodent studies are performed in house.
In vitro Toxicology
Cytotoxicity
- Test systems
Established cell lines and primary cells, 2D– monolayer, suspension and 3D-organoid cultures - Analysis
Fluorescence, luminescence, absorbance - Indicator
Resazurin, XTT, MTT, ATP, LDH - Readout
Viability, Cell Membrane Damage, Energy content
Mitochondrial Toxicity
- Test systems
HepG2, HepG2-Oxphos - Analysis
Fluorescence, luminescence - Indicator
Resazurin, XTT, ATP - Readout
Viability - Format
96-Well Plate/Plate Reader - Multiplexing of analytical endpoints available
Metabolite Toxicity
- Test systems
HepG2 w/ and w/o metabolic activation by S9-fraction - Analysis
Luminescence - Indicator
ATP - Format
96-Well Plate/Plate Reader - Metabolite identification via LC-MS
- Reactive metabolite trapping in liver microsomes
Immunotoxicity
- Test systems
PMBC, monocytes, blood - Analysis
Luminex technology - Readout
Lymphocyte subsets, cytokine levels - Format
96-Well Plate/Plate Reader, Luminex - Can be combined with other systems e.g. viability assays
- Recommended assays according to ICH S8
Hepatotoxicity
- Test systems
HepG2, primary hepatocytes - Analysis
Fluorescence, luminescence, Luminex technology - Indicator
Resazurin, ATP - Readout
Viability, Energy content, biomarker levels - Format
96-Well Plate/Plate Reader, Luminex
Allergic Response / Histamine Release
- Test systems
Peritoneal mastocytes - Analysis
Absorption - Indicator
O-phtaldialdehyde - Readout
Histamine release - Format
96-Well Plate/Plate Reader
In vivo Toxicology*
Species
- Rodents
- Non-rodent species
(please contact us)
Routes of Administration
- Oral
- Intraperitoneal
- Intravenous
- Subcutanous
- Dermal
- Inhalation
- Rectal
- Others
(please contact us)
Acute Toxicity
- Systemic toxicity
- Phototoxicity
- Local tolerance
- Skin sensitisation and irritation
- Others
(please contact us)
Subchronic & Chronic Toxicity
- Rodents and non-rodents
- DRF studies
- MTD studies
- Toxicokinetics
Reproductive Toxicity
- Multigeneration studies
- Fertility and reproduction
- Embryotoxicity
- Pre/post-natal development
Pathology
- Histology
- Immuno- histopathology
- others
(please contact us)
* in cooperation. All studies are conducted according to national and international guidelines.
Further toxicological assays are available on request.
Please contact us for your specific toxicology study!
For in vitro Toxicology
Dr. Julia Susewind
In vitro Toxicology
susewind@pharmacelsus.de
For in vivo Toxicology
Anika Christoffel
Team Manager in vivo Pharmacology & Pharmacokinetics