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Pharmacelsus

In vitro & in vivo Toxicology Services

The Pharmacelsus service portfolio on in vitro and in vivo toxicology service portfolio offers you a complete workflow. De-risk either your early stage drug discovery or late stage development programs with us. In vitro toxicology assays are performed in-house in a quality controlled way. In vivo toxicology studies according to national and international guidelines are available on rodent and non-rodent species in cooperation with our trusted partner. In vivo non-GLP rodent studies are performed in house.

In vitro Toxicology

Cytotoxicity

  • Test systems
    Established cell lines and primary cells, 2D– monolayer, suspension and 3D-organoid cultures
  • Analysis
    Fluorescence, luminescence, absorbance
  • Indicator
    Resazurin, XTT, MTT, ATP, LDH
  • Readout
    Viability, Cell Membrane Damage, Energy content

Mitochondrial Toxicity

  • Test systems
    HepG2, HepG2-Oxphos
  • Analysis
    Fluorescence, luminescence
  • Indicator
    Resazurin, XTT, ATP
  • Readout
    Viability
  • Format
    96-Well Plate/Plate Reader
  • Multiplexing of analytical endpoints available

Metabolite Toxicity

  • Test systems
    HepG2 w/ and w/o metabolic activation by S9-fraction
  • Analysis
    Luminescence
  • Indicator
    ATP
  • Format
    96-Well Plate/Plate Reader
  • Metabolite identification via LC-MS
  • Reactive metabolite trapping in liver microsomes

Immunotoxicity

  • Test systems
    PMBC, monocytes, blood
  • Analysis
    Luminex technology
  • Readout
    Lymphocyte subsets, cytokine levels
  • Format
    96-Well Plate/Plate Reader, Luminex
  • Can be combined with other systems e.g. viability assays
  • Recommended assays according to ICH S8

Hepatotoxicity

  • Test systems
    HepG2, primary hepatocytes
  • Analysis
    Fluorescence, luminescence, Luminex technology
  • Indicator
    Resazurin, ATP
  • Readout
    Viability, Energy content, biomarker levels
  • Format
    96-Well Plate/Plate Reader, Luminex

Allergic Response / Histamine Release

  • Test systems
    Peritoneal mastocytes
  • Analysis
    Absorption
  • Indicator
    O-phtaldialdehyde
  • Readout
    Histamine release
  • Format
    96-Well Plate/Plate Reader

In vivo Toxicology*

Species

  • Rodents
  • Non-rodent species
    (please contact us)

Routes of Administration

  • Oral
  • Intraperitoneal
  • Intravenous
  • Subcutanous
  • Dermal
  • Inhalation
  • Rectal
  • Others
    (please contact us)

Acute Toxicity

  • Systemic toxicity
  • Phototoxicity
  • Local tolerance
  • Skin sensitisation and irritation
  • Others
    (please contact us)

Subchronic & Chronic Toxicity

  • Rodents and non-rodents
  • DRF studies
  • MTD studies
  • Toxicokinetics

Reproductive Toxicity

  • Multigeneration studies
  • Fertility and reproduction
  • Embryotoxicity
  • Pre/post-natal development

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