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Pharmacelsus
In vitro & in vivo Toxicology Services
The Pharmacelsus service portfolio on in vitro and in vivo toxicology service portfolio offers you a complete workflow. De-risk either your early stage drug discovery or late stage development programs with us. In vitro toxicology assays are performed in-house in a quality controlled way. In vivo toxicology studies according to national and international guidelines are available on rodent and non-rodent species in cooperation with our trusted partner. In vivo non-GLP rodent studies are performed in house.
In vitro Toxicology
Cytotoxicity
- Test systems
Established cell lines and primary cells, 2D– monolayer, suspension and 3D-organoid cultures - Analysis
Fluorescence, luminescence, absorbance - Indicator
Resazurin, XTT, MTT, ATP, LDH - Readout
Viability, Cell Membrane Damage, Energy content
Mitochondrial Toxicity
- Test systems
HepG2, HepG2-Oxphos - Analysis
Fluorescence, luminescence - Indicator
Resazurin, XTT, ATP - Readout
Viability - Format
96-Well Plate/Plate Reader - Multiplexing of analytical endpoints available
Metabolite Toxicity
- Test systems
HepG2 w/ and w/o metabolic activation by S9-fraction - Analysis
Luminescence - Indicator
ATP - Format
96-Well Plate/Plate Reader - Metabolite identification via LC-MS
- Reactive metabolite trapping in liver microsomes
Immunotoxicity
- Test systems
PMBC, monocytes, blood - Analysis
Luminex technology - Readout
Lymphocyte subsets, cytokine levels - Format
96-Well Plate/Plate Reader, Luminex - Can be combined with other systems e.g. viability assays
- Recommended assays according to ICH S8
Hepatotoxicity
- Test systems
HepG2, primary hepatocytes - Analysis
Fluorescence, luminescence, Luminex technology - Indicator
Resazurin, ATP - Readout
Viability, Energy content, biomarker levels - Format
96-Well Plate/Plate Reader, Luminex
Allergic Response / Histamine Release
- Test systems
Peritoneal mastocytes - Analysis
Absorption - Indicator
O-phtaldialdehyde - Readout
Histamine release - Format
96-Well Plate/Plate Reader
In vivo Toxicology*
Species
- Rodents
- Non-rodent species
(please contact us)
Routes of Administration
- Oral
- Intraperitoneal
- Intravenous
- Subcutanous
- Dermal
- Inhalation
- Rectal
- Others
(please contact us)
Acute Toxicity
- Systemic toxicity
- Phototoxicity
- Local tolerance
- Skin sensitisation and irritation
- Others
(please contact us)
Subchronic & Chronic Toxicity
- Rodents and non-rodents
- DRF studies
- MTD studies
- Toxicokinetics
Reproductive Toxicity
- Multigeneration studies
- Fertility and reproduction
- Embryotoxicity
- Pre/post-natal development